- Cln Mutants
- cln1∆ cln2∆
- cln1∆ cln2∆ sic1∆
- cln1∆ cln2∆ cdh1∆
- cln1∆ cln2∆ bck2∆
- cln1∆ cln2∆ clb5∆ clb6∆
- cln1∆ cln2∆ cln3∆
- cln1∆ cln2∆ cln3∆ GAL-CLN2
- cln1∆ cln2∆ cln3∆ GAL-CLN3
- cln1∆ cln2∆ cln3∆ sic1∆
- cln1∆ cln2∆ cln3∆ cdh1∆
- cln1∆ cln2∆ cln3∆ multi-copy CLB5
- cln1∆ cln2∆ cln3∆ GAL-CLB5
- cln1∆ cln2∆ cln3∆ multicopy BCK2
- cln1∆ cln2∆ cln3∆ GAL-CLB2
- cln1∆ cln2∆ cln3∆ apc-ts
- GAL-SIC1 cln1∆ cln2∆
- GAL-SIC1 GAL-CLN2 cln1∆ cln2∆
- GAL-SIC1 cln1∆ cln2∆ cdh1∆
- GAL-SIC1 GAL-CLN2 cln1∆ cln2∆ cdh1∆
- GAL-CLN2 cln1∆ cln2∆ cdh1∆
- GAL-CLN2 cln1∆ cln2∆
- cdc14-ts GAL-CLN2
- cln3∆
- cln3∆ bck2∆
- cln3∆ bck2∆ GAL-CLN2 cln1∆ cln2∆
- cln3∆ bck2∆ multi-copy CLN2
- cln3∆ bck2∆ sic1∆
- GAL-CLN3
cln1∆ cln2∆ cln3∆ cdh1∆
debug: ,
test user =
test db =
Simulation:
Change of parameters: ksn2'= ksn2"=0, Dn3=0, kscdh=0, init CDH1=CDH1T=0.
Length of G1 phase: 200 min (requirement for G1 arrest is 201.9 min).
Arrest: Telophase.
Experiments:
Schwab, M., Lutum, A.S. and Seufert, W. (1997). Yeast Hct1 is a regulator of Clb2 cyclin proteolysis. Cell 90:683-693.
[Abstract] [Article]
[Abstract] [Article]
Experimental results: Fig. 3. "Although cdh1∆ cells are still sensitive to mating factor as they are unable to form colonies, these cells do not arrest uniformly in G1, a fraction of the cell population proceeds through S phase and arrests with 2C DNA content".
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Considering that the simulated cells just barely satisfy our condition for entering S phase, we might expect, in a population of real cells that vary around the mean simulated behavior, some cells will arrest with 1C DNA content and some with 2C DNA content, as observed by Schwab et al.